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To optimize SeNPs concentration for subsequent semen cryopreservation, three semen samples were individually split into four equals and diluted to a final concentration of 6.0 × 10 7 spermatozoa/ml with the basic extender supplemented with 0 μg/ml SeNPs, 1 μg/ml SeNPs, 2 μg/ml SeNPs, and 4 μg/ml SeNPs